DNA

Part:BBa_K1951003

Designed by: claire raynaud   Group: iGEM16_Aix-Marseille   (2016-10-10)


desD, desferrioxamine biosynthesis protein coding sequence

DesD, desferrionxamine biosynthesis protein

General information

Monooxygenase is an enzyme that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl and one H2O molecule by the concomitant oxidation of NAD(P)H. It is also the last step in the production of desferrioxamine B in Streptomyces coelicolor.

DesD in the Desferrionxamine B pathway

Design summary

All forbidden restriction sites have been removed.

Codons have been optimized for E.coli to allow a maximal transcription level.

Experiment summary

The protein production of DesD has been tested by our team in a composite parts we designed :

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 350
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1440
    Illegal BsaI.rc site found at 535


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Categories
Parameters
None